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Membrane Protein Preparation Methods for CryoEM 

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Ras and PI3K Pathways in Cancer

medical illustration of Two approaches to membrane protein preparation for cryo-electron microscopy (cryoEM) shown in schematic diagram format, including the GraDeR technique for excess detergent removal and nanodiscs.
Next medical illustration of Schematic diagram of the catalytic cycle of multidrug transporter P-glycoprotein (P-gp, ABCB1). PGP hydrolyzes ATP in order to remove various drugs from the cell, and plays a role in drug resistance. 

This diagram summarizes findings from multiple cryo-electron microscopy (cryo-EM) structures.

Human P-glycoprotein (PGP) Catalytic Cycle

Two approaches to membrane protein preparation for cryo-electron microscopy (cryoEM) shown in schematic diagram format, including the GraDeR technique for excess detergent removal and nanodiscs.

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