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Falconieri Visuals

Cryo-Electron Microscopy: Technique Overview 

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Receptor Tyrosine Kinase Flt-1 binds ligand PlGF

Next medical illustration of Subject:  Nanoscopic 3D data of mouse salivary cells, salivary acinus anatomy
Objective: Highlight the capabilities of focused ion beam scanning electron microscopy (FIB-SEM) in studying nanoscale biological features, using the example of a mouse salivary gland. These capabilities include not only 3D structural data, but many individual 2D images that capture multiple instances of a process, as demonstrated by the inset of the 3 stages of salivary vesicle fusion.

A Spitting Image in 3D

Video overview of cryo-EM technique, from purified protein solution to 3D structure. Cryo-electron microscopy (cryo-EM) is an emerging structural biology method that recently achieved atomic resolution. This resolution is on par with the more traditional technique of X-ray crystallography. Cryo-EM now makes it possible to study a wider range of 3D molecular structures at high resolution. This video version was created in collaboration with Science Magazine (narration, content cut direction)

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