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Genomewide Genetic Perturbation Screening. 

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Metagenomic sequencing

medical illustration of Library of gRNA is introduced into cells containing Cas9. RelA, which normally is located in the cytoplasm, is induced to move into the nucleus.. The stimulated cells are then sorted on the basis of correlation between the RelA intensity signal and a fluorescent nuclear stain. The two cell populations undergo sequencing for detection of gRNA. N Engl J Med 2022; 386:1755-1758 DOI: 10.1056/NEJMcibr2200971
Next medical illustration of One of a series of illustrations accompanying an article on xenotransplantation. This figure demonstrates the process of somatic cell nuclear transfer which permits derivation of genetically-modified animals, such as pigs. That process, in combination with additional ‘knock-in’ and ‘knock-out’ genes can produce organs that are less likely to elicit hyperimmune acute rejection when transferred to human recipients. N Engl J Med 2022;387:35-44.

From somatic cell to genetically-modified pig

Library of gRNA is introduced into cells containing Cas9. RelA, which normally is located in the cytoplasm, is induced to move into the nucleus.. The stimulated cells are then sorted on the basis of correlation between the RelA intensity signal and a fluorescent nuclear stain. The two cell populations undergo sequencing for detection of gRNA. N Engl J Med 2022; 386:1755-1758 DOI: 10.1056/NEJMcibr2200971

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